Synergistic corrosion effects of magnetite and microorganisms: microbial community dependency.

The synergistic corrosion effect of acid-producing bacteria (APB) and magnetite on carbon steel corrosion was assessed using two different microbial consortia. A synergistic corrosion effect was observed exclusively with Consortium 2, which was composed of Enterobacter sp., Pseudomonas sp., and Tepidibacillus sp. When Consortium 2 was accompanied by magnetite, uniform corrosion and pitting rates were one-time higher (0.094 mm/year and 0.777 mm/year, respectively) than the sum of the individual corrosion rates promoted by the consortium and deposit separately (0.084 and 0.648 mm/year, respectively). The synergistic corrosion effect observed exclusively with Consortium 2 is attributed to its microbial community structure. Consortium 2 exhibited higher microbial diversity that benefited the metabolic status of the community. Although both consortia induced acidification of the test solution and metal surface through glucose fermentation, heightened activity levels of Consortium 2, along with increased surface roughness caused by magnetite, contributed to the distinct synergistic corrosion effect observed with Consortium 2 and magnetite. KEY POINTS: • APB and magnetite have a synergistic corrosion effect on carbon steel. • The microbial composition of APB consortia drives the synergistic corrosion effect. • Magnetite increases carbon steel surface roughness.

Degradation and lifetime prediction of plastics in subsea and offshore infrastructures.

Engineering and civil developments have relied on synthetic polymers and plastics (including polyethylene, polypropylene, polyamide, etc.) for decades, especially where their durability protects engineering structures against corrosion and other environmental stimuli. Offshore oil and gas infrastructure and renewable energy platforms are typical examples, where these plastics (100,000 s of metric tonnes worldwide) are used primarily as functional material to protect metallic flowlines and subsea equipment against seawater corrosion. Despite this, the current literature on polymers is limited to sea-surface environments, and a model for subsea degradation of plastics is needed. In this review, we collate relevant studies on the degradation of plastics and synthetic polymers in marine environments to gain insight into the fate of these materials when left in subsea conditions. We present a new mathematical model that accounts for various physicochemical changes in the oceanic environment as a function of depth to predict the lifespan of synthetic plastics and the possible formation of plastic debris, e.g., microplastics. We found that the degradation rate of the plastics decreases significantly as a function of water depth and can be estimated quantitatively by the mathematical model that accounts for the effect (and sensitivity) of geographical location, temperature, light intensity, hydrostatic pressure, and marine sediments. For instance, it takes a subsea polyethylene coating about 800 years to degrade on ocean floor (as opposed to <400 years in shallow coastal waters), generating 1000s of particles per g of degradation under certain conditions. Our results demonstrate how suspended sediments in the water column are likely to compensate for the decreasing depth-corrected degradation rates, resulting in surface abrasion and the formation of plastic debris such as microplastics. This review, and the complementing data, will be significant for the environmental impact assessment of plastics in subsea infrastructures. Moreover, as these infrastructures reach the end of their service life, the management of the plastic components becomes of great interest to environmental regulators, industry, and the community, considering the known sizeable impacts of plastics on global biogeochemical cycles.

Effect of cetrimonium carrier micelles on bacterial membranes and extracellular DNA, an in silico study.

Microorganisms do not live as dispersed single cells but rather they form aggregates with extracellular polymeric substances at interfaces. Biofilms are considered efficient life forms because they shield bacteria from biocides and collect dilute nutrients. This is a big concern in industry since the microorganisms can colonize a wide range of surfaces, accelerating material deterioration, colonizing medical devices, contaminating ultrapure drinking water, increasing energy costs and creating focus of infection. Conventional biocides that target a specific component of the bacteria are not effective in the presence of biofilms. Efficient biofilm inhibitors are based on a multitarget approach interacting with the bacteria and the biofilm matrix. Their rationale design requires a thorough understanding of inhibitory mechanisms that are still largely lacking today. Herein we uncover via molecular modelling the inhibition mechanism of cetrimonium 4-OH cinnamate (CTA-4OHcinn). Simulations show that CTA-4OH micelles can disrupt symmetric and asymmetric bilayers, representative of inner and outer bacterial membranes, following three stages: adsorption, assimilation, and defect formation. The main driving force for micellar attack is electrostatic interactions. In addition to disrupting the bilayers, the micelles work as carriers facilitating the trapping of 4OH cinnamate anions within the bilayer upper leaflet and overcoming electrostatic repulsion. The micelles also interact with extracellular DNA (e-DNA), which is one of the main components of biofilms. It is observed that CTA-4OHcinn forms spherical micelles on the DNA backbone; which hinders their ability to pack. This is demonstrated by modelling the DNA along the hbb histone-like protein, showing that in the presence of CTA-4OHcinn, DNA does not pack properly around hbb. The abilities of CTA-4OHcinn to cause cell death through membrane disruption and to disperse a mature, multi-species biofilm are also confirmed experimentally.

Effect of deposit chemistry on microbial community structure and activity: Implications for under-deposit microbial corrosion.

Introduction: The deposition of solid particles carried by production fluids from oil and gas companies in horizontal surfaces of different assets has shown to cause severe localised corrosion. Sand, one of the most common deposits in the energy sector pipelines, is frequently mixed with crude, oil, asphaltenes, corrosion inhibitors, and other organic compounds. For this reason, they might favour the metabolic activity of native microbial communities. This study aimed to determine the impact of sand-deposit chemical composition on the microbial community structure and functional attributes of a multispecies consortium recovered from an oilfield and the resulting risk of under-deposit microbial corrosion of carbon steel. Methods: Sand deposits recovered from an oil pipeline were used in their raw form and compared against the same deposits exposed to heat treatment to remove organic compounds. A four-week immersion test in a bioreactor filled with synthetic produced water and a two-centimeter layer of sand was set up to assess corrosion and microbial community changes. Results: The raw untreated deposit from the field containing hydrocarbons and treatment chemicals resulted in a more diverse microbial community than its treated counterpart. Moreover, biofilms developed in the raw sand deposit exhibited higher metabolic rates, with functional profile analysis indicating a predominance of genes associated with xenobiotics degradation. Uniform and localized corrosion were more severe in the raw sand deposit compared to the treated sand. Discussion: The complex chemical composition of the untreated sand might have represented an additional source of energy and nutrients to the microbial consortium, favoring the development of different microbial genera and species. The higher corrosion rate obtained under the untreated sand suggests that MIC occurred due to syntrophic relationships between sulphate reducers or thiosulphate reducers and fermenters identified in the consortium.

Extracellular DNA: A Critical Aspect of Marine Biofilms.

Multispecies biofilms represent a pervasive threat to marine-based industry, resulting in USD billions in annual losses through biofouling and microbiologically influenced corrosion (MIC). Biocides, the primary line of defence against marine biofilms, now face efficacy and toxicity challenges as chemical tolerance by microorganisms increases. A lack of fundamental understanding of species and EPS composition in marine biofilms remains a bottleneck for the development of effective, target-specific biocides with lower environmental impact. In the present study, marine biofilms are developed on steel with three bacterial isolates to evaluate the composition of the EPSs (extracellular polymeric substances) and population dynamics. Confocal laser scanning microscopy, scanning electron microscopy, and fluorimetry revealed that extracellular DNA (eDNA) was a critical structural component of the biofilms. Parallel population analysis indicated that all three strains were active members of the biofilm community. However, eDNA composition did not correlate with strain abundance or activity. The results of the EPS composition analysis and population analysis reveal that biofilms in marine conditions can be stable, well-defined communities, with enabling populations that shape the EPSs. Under marine conditions, eDNA is a critical EPS component of the biofilm and represents a promising target for the enhancement of biocide specificity against these populations.

Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption.

Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems.

Corrosion of Carbon Steel by Shewanella chilikensis DC57 Under Thiosulphate and Nitrate Reducing Conditions.

Shewanella chilikensis DC57 is a bacterial strain isolated from a corrosion failure in a floating oil production system. Previous studies have indicated that this microorganism has potential to trigger corrosion of carbon steel through several metabolic pathways identified in its genome. In this study we evaluated the corrosion of carbon steel by S. chilikensis in the presence of thiosulphate or nitrate as terminal electron acceptors of the anaerobic respiration. Electrochemical response of carbon steel to the biofilm formation revealed differences in the corrosion process under the different electron acceptors conditions. Microscopic examination of the metal surface confirmed that S. chilikensis induced corrosion in both scenarios; however, in the presence of thiosulfate S. chilikensis triggered a higher pitting corrosion rate, whereas in presence of nitrate it promoted higher uniform corrosion. This study demonstrates the importance of understanding the metabolic versatility of microbes in order to assess the MIC risk of industrial facilities.

Conditioning of metal surfaces enhances Shewanella chilikensis adhesion.

Microbiologically influenced corrosion and biofouling of steels depend on the adsorption of a conditioning film and subsequent attachment of bacteria. Extracellular deoxyribonucleic acid (eDNA) and amino acids are biologically critical nutrient sources and are ubiquitous in marine environments. However, little is known about their role as conditioning film molecules in early biofilm formation on metallic surfaces. The present study evaluated the capacity for eDNA and amino acids to form a conditioning film on carbon steel (CS), and subsequently, the influence of these conditioning films on bacterial attachment using a marine bacterial strain. Conditioning films of eDNA or amino acids were formed on CS through physical adsorption. Biochemical and microscopic analysis of eDNA conditioning, amino acid conditioning and control CS surfaces demonstrated that organic conditioning surfaces promoted bacterial attachment. The results highlight the importance of conditioning the surface in initial bacterial attachment to steel.

Efficiency of a Novel Multifunctional Corrosion Inhibitor Against Biofilms Developed on Carbon Steel.

In natural environments, populations of microorganisms rapidly colonise surfaces forming biofilms. These sessile communities comprise a variety of species which contribute to biofouling and microbiologically influenced corrosion (MIC), especially on metals. Species heterogeneity in natural systems confers higher tolerance to adverse conditions such as biocide treatment compared with single species laboratory simulations. Effective chemical treatments to combat recalcitrant biofilms are often dangerous to apply; both to operators and the environment, and face international embargoes. Today, there is a drive to exchange current toxic and environmentally hazardous biocides with less harmful compounds. One effective method of achieving this goal is to generate multi-functional compounds capable of tackling corrosion and biofilm formation simultaneously, thus reducing the number of compounds in dosing procedures. In a previous study, a novel corrosion inhibitor demonstrated biocidal effects against three marine isolates during the early stages of biofilm formation. The compound; CTA-4OHcinn, holds great promise as a multi-functional inhibitor, however its effect on complex, multi-species biofilms remains unknown. Here we evaluate CTA-4OHcinn biocidal capacity against multi-species biofilms developed from oilfield samples. Mature biofilms were developed and treated with 10 mM CTA-4OHcinn for 4 h. The effects of the compound were assessed using mean probable number (MPN), adenosine triphosphate (ATP) quantification, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Results demonstrate that CTA-4OHcinn significantly reduces the viability of mature biofilms, supporting previous demonstrations on the secondary function of CTA-4OHcinn as a biocide. CLSM results further indicate that CTA-4OHcinn targets the cell membrane resulting in lysis. This finding complements the established corrosion inhibition function of CTA-4OHcinn, indicating the compound is a true multi-functional organic corrosion inhibitor.

Evaluation of a novel, multi-functional inhibitor compound for prevention of biofilm formation on carbon steel in marine environments.

Chemical biocides remain the most effective mitigation strategy against microbiologically influenced corrosion (MIC), one of the costliest and most pervasive forms of corrosion in industry. However, toxicity and environmental concerns associated with these compounds are encouraging the development of more environmentally friendly MIC inhibitors. In this study, we evaluated the antimicrobial effect of a novel, multi-functional organic corrosion inhibitor (OCI) compound, cetrimonium trans-4-hydroxy-cinnamate (CTA-4OHcinn). Attachment of three bacterial strains, Shewanella chilikensis, Pseudomonas balearica and Klebsiella pneumoniae was evaluated on wet-ground (120 grit finish) and pre-oxidised carbon steel surfaces (AISI 1030), in the presence and absence of the new OCI compound. Our study revealed that all strains preferentially attached to pre-oxidised surfaces as indicated by confocal laser scanning microscopy, scanning electron microscopy and standard colony forming unit (CFU) quantification assays. The inhibitor compound at 10 mM demonstrated 100% reduction in S. chilikensis attachment independent of initial surface condition, while the other two strains were reduced by at least 99.7% of the original viable cell number. Our results demonstrate that CTA-4OHcinn is biocidal active and has promise as a multifunctional, environmentally sound MIC inhibitor for industrial applications.

Electrochemical and Surface Characterization Study on the Corrosion Inhibition of Mild Steel 1030 by the Cationic Surfactant Cetrimonium Trans-4-hydroxy-cinnamate.

Effective corrosion inhibition of mild steel 1030 at 0.01 M NaCl concentration was achieved by the use of the nontoxic surfactant salt cetrimonium trans-4-hydroxy-cinnamate (CTA-4OHcinn). Polarization analysis on the steel samples immersed for 24 h in the control and CTA-4OHcinn-containing solutions shows the development of a passivation potential that is more obvious at higher inhibitor concentrations along with a maximum inhibition efficiency of 97.8%. Electrochemical impedance spectroscopy (EIS) pinpoints the effect of the inhibitor on the corroding regions of the metal surface, showing an increase in the local electric resistance and conversely a decrease in the local capacitance, which indicates that the charge transfer in the corroding regions is being hindered by a deposition process. This is consistent with scanning electron microscopy (SEM) images, showing the presence of a porous oxide matrix that fills localized corrosion sites on the metal surface after 24 h of immersion in a 0.01 M NaCl + 10 mM inhibitor solution. Additionally, SEM analysis also shows the formation of an organic film surrounding the defects that is able to shield chloride attack. As a result of diffusion of chloride from the defects below the protective film, filiform corrosion can be seen. Time-resolved impedance analysis over the first 120 min of immersion in the control and inhibitor solution shows that significant inhibitor protection does not take place immediately and there is a lag phase in the first 50 min of immersion, suggesting that early localized corrosion drives further adsorption of inhibitor micelles on the metal surface. This is in agreement with X-ray photoelectron spectroscopy (XPS) analysis, which indicates a complete surface coverage over the first 2 h of immersion in a concentrated inhibitor solution. XPS also shows the heterogeneity of the film, where some parts are poorly covered, revealing the underlying surface containing iron.

In Situ Investigation of Under-Deposit Microbial Corrosion and its Inhibition Using a Multi-Electrode Array System.

Carbon steel pipelines used in the oil and gas industry can be susceptible to the combined presence of deposits and microorganisms, which can result in a complex phenomenon, recently termed under-deposit microbial corrosion (UDMC). UDMC and its inhibition in CO2 ambiance were investigated in real-time using a multi-electrode array (MEA) system and surface profilometry analysis. Maps from corrosion rates, galvanic currents, and corrosion potentials recorded at each microelectrode allowed the visualization of local corrosion events on the steel surface. A marine bacterium Enterobacter roggenkampii, an iron-oxidizing, nitrate-reducing microorganism, generated iron deposits on the surface that resulted in pitting corrosion under anaerobic conditions. Areas under deposits displayed anodic behavior, more negative potentials, higher corrosion rates, and pitting compared to areas outside deposits. In the presence of the organic film-forming corrosion inhibitor, 2-Mercaptopyrimidine, the marine bacterium induced local breakdown of the protective inhibitor film and subsequent pitting corrosion of carbon steel. The ability of the MEA system to locally measure self-corrosion processes, galvanic effects and, corrosion potentials across the surface demonstrated its suitability to detect, evaluate and monitor the UDMC process as well as the efficiency of corrosion inhibitors to prevent this corrosion phenomenon. This research highlights the importance of incorporating the microbial component to corrosion inhibitors evaluation to ensure chemical effectiveness in the likely scenario of deposit formation and microbial contamination in oil and gas production equipment.

Complete Genome Sequence of Shewanella chilikensis Strain DC57, Isolated from Corroded Seal Rings at a Floating Oil Production System in Australia.

Here, we describe the genome of Shewanella chilikensis strain DC57, a facultatively anaerobic bacterium isolated from corroded seal rings at a floating oil production system in Australia. The genome of strain DC57 has a size of 4.91 Mbp and harbors 4,178 predicted protein-encoding genes.

Draft Genome Sequence of Enterobacter roggenkampii Strain OS53, Isolated from Corroded Pipework at an Offshore Oil Production Facility.

Here, we report the genome sequence of Enterobacter roggenkampii strain OS53, isolated from corroded pipework at an offshore oil production facility. The draft genome sequence comprises 6 contigs and contains 5,194,507 bp with an average GC content of 55.90%.

Carbon steel corrosion by bacteria from failed seal rings at an offshore facility.

Corrosion of carbon steel by microorganisms recovered from corroded seal rings at an offshore floating production facility was investigated. Microbial diversity profiling revealed that communities in all sampled seal rings were dominated by Pseudomonas genus. Nine bacterial species, Pseudomonas aeruginosa CCC-IOB1, Pseudomonas balearica CCC-IOB3, Pseudomonas stutzeri CCC-IOB10, Citrobacter youngae CCC-IOB9, Petrotoga mobilis CCC-SPP15, Enterobacter roggenkampii CCC-SPP14, Enterobacter cloacae CCC-APB1, Cronobacter sakazakii CCC-APB3, and Shewanella chilikensis CCC-APB5 were isolated from corrosion products and identified based on 16S rRNA gene sequence. Corrosion rates induced by the individual isolates were evaluated in artificial seawater using short term immersion experiments at 40 °C under anaerobic conditions. P. balearica, E. roggenkampii, and S. chilikensis, which have not been associated with microbiologically influenced corrosion before, were further investigated at longer exposure times to better understand their effects on corrosion of carbon steel, using a combination of microbiological and surface analysis techniques. The results demonstrated that all bacterial isolates triggered general and localised corrosion of carbon steel. Differences observed in the surface deterioration pattern by the different bacterial isolates indicated variations in the corrosion reactions and mechanisms promoted by each isolate.

Complete Genome Sequence of Pseudomonas balearica Strain EC28, an Iron-Oxidizing Bacterium Isolated from Corroded Steel.

Pseudomonas balearica strain EC28 is an iron-oxidizing bacterium isolated from corroded steel at a floating production storage and offloading facility in Australia. Here, we report its complete genome sequence, which comprises 4,642,566 bp with a GC content of 64.43%. The genome harbors 4,164 predicted protein-encoding genes.

Nutrient Level Determines Biofilm Characteristics and Subsequent Impact on Microbial Corrosion and Biocide Effectiveness.

The impact that nutrient level has on biofilm characteristics, biocide effectiveness, and the associated risk of microbiologically influenced corrosion (MIC) was assessed using multispecies biofilms from two different oilfield consortia. A range of microbiological, microscopy, and corrosion methods demonstrated that the continuous flow of nutrients for the microbial growth resulted in higher activity, thickness, and robustness of the biofilms formed on carbon steel, which induced greater localized corrosion compared to biofilms formed under batch, nutrient-depleted conditions. Despite of the differences in biofilm characteristics, biofilms displayed comparable susceptibilities to glutaraldehyde biocide, with similar log10 reductions and percent reductions of microorganisms under both nutrient conditions. Nevertheless, nutrient replenishment impacted the effectiveness of the biocide in controlling microbial populations; a higher concentration of cells survived the biocide treatment in biofilms formed under a continuous flow of nutrients. Complementary DNA-/RNA-based amplicon sequencing and bioinformatics analysis were used to discriminate the active within the total populations in biofilms established at the different nutrient conditions and allowed the identification of the microbial species that remained active despite nutrient depletion and biocide treatment. Detection of persistent active microorganisms after exposure to glutaraldehyde, regardless of biofilm structure, suggested the presence of microorganisms less susceptible to this biocide and highlighted the importance of monitoring active microbial species for the early detection of biocide resistance in oil production facilities.IMPORTANCE Microbiologically influenced corrosion (MIC) is a complex process that generates economic losses to the industry every year. Corrosion must be managed to prevent a loss of containment of produced fluids to the external environment. MIC management includes the identification of assets with higher MIC risk, which could be influenced by nutrient levels in the system. Assessing biofilms under different nutrient conditions is essential for understanding the impact of flow regime on microbial communities and the subsequent impact on microbial corrosion and on the effectiveness of biocide treatment. This investigation simulates closely oil production systems, which contain piping sections exposed to continuous flow and sections that remain stagnant for long periods. Therefore, the results reported here are useful for MIC management and prevention. Moreover, the complementary methodological approach applied in this investigation highlighted the importance of implementing RNA-based methods for better identification of active microorganisms that survive stress conditions in oil systems.

Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an Oil Production Facility.

DNA and RNA-based sequencing of the 16S rRNA gene and transcripts were used to assess the phylogenetic diversity of microbial communities at assets experiencing corrosion in an oil production facility. The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualize differences between the total and potentially active communities present in several locations of the production facility. According to the results, taxa indicative for thermophiles and oil-degrading microorganisms decreased their relative abundances in the active communities, whereas sulfate reducing bacteria and methanogens had the opposite pattern. The differences in the diversity profile between total and active communities had an effect on the microbial functional capability predicted from the 16S rRNA sequences. Primarily, genes involved in methane metabolism were enriched in the RNA-based sequencing approach. Comparative analysis of microbial communities in the produced water, injection water and deposits in the pipelines showed that deposits host more individual species than other sample sources in the facility. Similarities in the number of cells and microbial profiles of active communities in biocide treated and untreated sampling locations suggested that the treatment was ineffective at controlling the growth of microbial populations with a known corrosive metabolism. Differences in the results between DNA and RNA-based profiling demonstrated that DNA results alone can lead to the underestimation of active members in the community, highlighting the importance of using a complementary approach to obtain a broad general overview not only of total and active members but also in the predicted functionality.

Effect of Quorum Sensing on the Ability of Desulfovibrio vulgaris To Form Biofilms and To Biocorrode Carbon Steel in Saline Conditions.

Sulfate-reducing bacteria (SRB) are key contributors to microbe-induced corrosion (MIC), which can lead to serious economic and environmental impact. The presence of a biofilm significantly increases the MIC rate. Inhibition of the quorum-sensing (QS) system is a promising alternative approach to prevent biofilm formation in various industrial settings, especially considering the significant ecological impact of conventional chemical-based mitigation strategies. In this study, the effect of the QS stimulation and inhibition on Desulfovibrio vulgaris is described in terms of anaerobic respiration, cell activity, biofilm formation, and biocorrosion of carbon steel. All these traits were repressed when bacteria were in contact with QS inhibitors but enhanced upon exposure to QS signal molecules compared to the control. The difference in the treatments was confirmed by transcriptomic analysis performed at different time points after treatment application. Genes related to lactate and pyruvate metabolism, sulfate reduction, electron transfer, and biofilm formation were downregulated upon QS inhibition. In contrast, QS stimulation led to an upregulation of the above-mentioned genes compared to the control. In summary, these results reveal the impact of QS on the activity of D. vulgaris, paving the way toward the prevention of corrosive SRB biofilm formation via QS inhibition.IMPORTANCE Sulfate-reducing bacteria (SRB) are considered key contributors to biocorrosion, particularly in saline environments. Biocorrosion imposes tremendous economic costs, and common approaches to mitigate this problem involve the use of toxic and hazardous chemicals (e.g., chlorine), which raise health and environmental safety concerns. Quorum-sensing inhibitors (QSIs) can be used as an alternative approach to inhibit biofilm formation and biocorrosion. However, this approach would only be effective if SRB rely on QS for the pathways associated with biocorrosion. These pathways would include biofilm formation, electron transfer, and metabolism. This study demonstrates the role of QS in Desulfovibrio vulgaris on the above-mentioned pathways through both phenotypic measurements and transcriptomic approach. The results of this study suggest that QSIs can be used to mitigate SRB-induced corrosion problems in ecologically sensitive areas.

Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1.

In this work, the biodegradation of the crude oil by the potential biosurfactant producing Bacillus subtilis A1 was investigated. The isolate had the ability to synthesize degradative enzymes such as alkane hydroxylase and alcohol dehydrogenase at the time of biodegradation of hydrocarbon. The biosurfactant producing conditions were optimized as pH 7.0, temperature 40°C, 2% sucrose and 3% of yeast extract as best carbon and nitrogen sources for maximum production of biosurfactant (4.85 g l-1). Specifically, the low molecular weight compounds, i.e., C10-C14 were completely degraded, while C15-C19 were degraded up to 97% from the total hydrocarbon pools. Overall crude oil degradation efficiency of the strain A1 was about 87% within a short period of time (7 days). The accumulated biosurfactant from the biodegradation medium was characterized to be lipopeptide in nature. The strain A1 was found to be more robust than other reported biosurfactant producing bacteria in degradation efficiency of crude oil due to their enzyme production capability and therefore can be used to remove the hydrocarbon pollutants from contaminated environment.